Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Data were expressed as mean percentage of viable cells. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. ). Awareness Technology, Inc. These isotypes cause antigen cross-linking by multimeric binding and self. GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. View the Molecular Devices website. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Data were expressed as the mean percent viable cell vs. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 1: SpectraMax Plus 384 components. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. 0 OD. , Sunnyvale, CA). 3. Gerritsma,B. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. A. Binding of capture antibodies to polystyrene beads and biotinylation. Acceptor: 665/10 nm. Percent Transmission Absorbance Competitor 0. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. MCE has not independently confirmed the accuracy of these methods. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 2. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Briefly, cells were seeded into a. 2. 5. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). StakMax. As a common indicator of equine T cell activation, cytokine induction. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. Molecular Devices SpectraMax M5e Microplate Reader. Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. Products : SpectraMax Plus 384 reader,. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). 2 Chemical Reagents 1. 04. Although all samples initially were analyzed at the same volume, the volume of sample. 1. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). Stat Fax 4700 Microstrip Reader. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. 2 were considered negative for IgM antibody. Six wells were assayed for each set of conditions and stadard deviations. Microplate Readers. 0 to 4. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Molecular Devices Spectramax 340PC instructions. What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full bandwidth at 1/2 the transmission peak for the selected wavelength. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. , Sunnyvale, CA). ac. used. Manager: Yinghua Chen, PhD Contact Email: yinghua. 1% N-1-naphtylethylenediamine dihydrochloride, 2. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. 0 1. TGFα release was calculated as described in the Results. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. They are for reference only. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Emission. The. Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). 94 g calcium chloride dihydrate (both. 024). Upgradeable modules are also available including western blot, cell imaging, and fast. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. ). Immunofluorescence and Aggregation Analysis. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The microtiter plate was then incubated in the dark for 2 h at 37°C. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. Spectrophotometer (SpectraMax 340). Call 1-800-745-2710. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. Exhibit with us {{>currencyLabel}} Back. Detection limits. Each experiment was performed in duplicate and repeated at. Softmax PRO v5. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Labsystems Luminoskan RT Microplate Reader. Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. Each experiment was performed in duplicate. Unit has several areas of damaged plastic. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Near bottom. The plates were incubated for 4 additional hours at 37°C. The growth. Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. plates can be used in the SPECTRAmax PLUS 384. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 0 2. They are for reference only. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Molecular Device M2e. To correct for inter. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Wavelength Selection. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. 314 low lamp level warning. SpectraMax Multi-Mode Microplate Readers. 303 unable to cal dark current. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. VersaMax reads 96-well and strip-well plates. These robust readers have been placed in labs from Antarctica to the. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Subsequently, the mixture was heated at 100 °C for 10. The ratio of red to green fluorescence intensity was calculated. 2. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. Glutathione reductase (GR) activity was measured, according to Smith et al. Plates coated with no antigen, canine CD19, streptavidin alone, and streptavidin with irrelevant human avitag-CD3ε (Acro Biosystems, Newark, DE) were used as negative controls. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. Molecular Devices SpectraMax Gemini XS Microplate Reader. The inhibition percentage was determined by linear regression through ranges of 20 to 30. Values were determined according to a standard curve (log/log curve-fit). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. . SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . Samples were plated in duplicate (r=0. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Samples containing 25 μg of protein were separated on 12. Create custom fluorescence assays without having to purchase expensive filter sets. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. 315 can't find zero order. Immunofluorescence and Aggregation Analysis. MCE has not independently confirmed the accuracy of these methods. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. Inter-assay variation was prevented by testing all of the samples on the same day. SpectraMAX 340 microtiter plate reader. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Heme was preincubated for 5 min with A when added before the metals. 5. These models can no longer be repaired. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. Vessel Sprout Formation Assay. The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. The area under the curve (AUC) for the comparison of the growth rates was determined using. Summary of Contents for Molecular Devices SPECTRAmax 340PC. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. TGFα release was calculated as described in the Results. Tunable for filterless flexibility. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. 316 grating motor driver faulty. 0. SpectraMax 250 With Computer SoftwareSpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. View the Molecular Devices website. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Driessen*,MaaikeA. 3. , Sunnyvale, CA, USA) at a wavelength of 450 nm. SpectraMAX 340. 4) was followed according to Vanacker et al. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). TDP-43 stress granular cells were treated with 70% ice-cold. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Values were determined according to a standard curve (log/log curve-fit). SPECTRAmax® 190. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. Water – distilled water or equivalent. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. These lab workhorses are used in drug discovery, research, bioassay validation, and. #N207) LAL Reagent Water (Lonza cat. Stat Fax 4700 Microstrip Reader. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. 81. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. Near bottom. The assays contained 0. \r plates can be used in the SPECTRAmax PLUS 384. Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. Data System. 5 mL sample or standard solution was well mixed with 0. Molecular Devices SpectraMax Gemini XS Microplate Reader. 0% and ± 0. 340 nm. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. The slope of the linear portion of the kinetic curve generated from each well was used to. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Pharmacokinetic and pharmacodynamic analysis. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 2 software was used to measure and calculate of the bioactivity activity studies. SpectraMAX 340. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. Donor filter: 616/10 nm. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). Microplate Reader Shelf. 30. 5. The Catalina. 30. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. A. Molecular Devices Corporation 1311. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. Subsequent replacements require only the lamp (N-34-LB) be replaced. Cell proliferation was measured using the MTT method. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. 7 macrophages were obtained from ATCC (Manassas, VA). NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. SpectraMax Multi-Mode Microplate Readers. The SpectraMax® iD3 Multi Mode Microplate . This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. SPECTRAmax® 190. ): 8. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. 2. SpectraMax Multi-Mode Microplate Readers. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. The SPECTRAmax 340PC can mea- TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. SpectraMax Multi-Mode Microplate Readers. Homeostatic model assessment for insulin. SpectraMax Multi-Mode Microplate Readers. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hIranian Journal of Basic Medical Sciences ijbms. Read one sample or up to 384. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. Triton X-100 (see Note 2). Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. 5 stars - 1602 reviewsThis assay typically develops in 1. One plate carrier adapter is provided with the instrument. TGFα release was calculated as described in the Results. Hydrolysis of compounds 9-15 was monitoredAbsorption was measured at 405 nm against 490 nm using a microplate reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. Details. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. 314 low lamp level warning. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. 306 plate air cal. Setup recommendations for SpectraMax. chen@case. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. 30. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Immunofluorescence and Aggregation Analysis. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. Microplate Readers. D2. or microplate reader (e. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Assay setup for a 384-well low-volume plate. 1% PEG 8000 at 37°C. 5. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). using SpectraMax i3 (Molecular devices, San Jose, USA). With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Revvity. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. The absorbances were being measured at 515 nm. One plate carrier adapter is provided with the instrument. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 30. Cross sections of mouse skin was prepared and stained as previously described . A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. #N207) LAL Reagent Water (Lonza cat. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. SpectraMax Multi-Mode Microplate Readers. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. 0. used in the SPECTRAmax PLUS. control. Other technologies such as imaging. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. Male 8 pin mini Din to Female 9D. Both readers feature an 8-channel read head for fast reads and. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. Briefly, cells were seeded into a. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. Bertin Precellys Evolution Touch Homogenizer. PerkinElmer. 6. All data were normalized to experimental day 0. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. The absorbance at 405 nm was determined using a microplate reader (spectraMAX 340). Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. 00. MSD read buffer and TMB one component HRP substrate are from MSD and SurModics (Eden Prairile, MN), respectively. The reaction was stopped with 1 M hydrochloric acid after 15 min. Dissolve 13. Description. Description 1. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SPECTRAmax 340PC can measure. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or.